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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-28
Proteomic Analysis of Sebocytes Co-Cultured with Mesenchymal Stem Cells
(CNU)
oMaryam Adelipour, Jeongkwon Kim
Sebocytes, specialized epithelial cells in sebaceous glands, play a key role in skin homeostasis by producing sebum, a lipid-rich substance essential for hydration, thermoregulation, and antimicrobial defense. Dysfunction in sebocyte activity, including altered lipid composition or excessive sebum production, is linked to dermatological conditions such as acne vulgaris and seborrheic dermatitis. Mesenchymal stem cells (MSCs) possess immunomodulatory and anti-inflammatory properties, making them promising candidates for regenerative medicine. This study investigated MSC-induced changes in the sebocyte proteome using mass spectrometry-based proteomics. Sebocytes were cultured in Sebomed medium and co-cultured with MSCs using transwell inserts for 48 hours. After incubation, cells were lysed, and proteins were extracted using the S-Trap method. Proteomic profiling via label-free quantification in MaxQuant identified 946 proteins, 78 of which were differentially expressed (41 upregulated, 37 downregulated). Upregulated proteins were associated with translation initiation and ubiquitin-protein transferase activity, while downregulated proteins were linked to chromatin DNA binding. These findings suggest that MSCs modulate sebocyte proteome dynamics, potentially influencing their function. Further research is needed to elucidate the molecular mechanisms underlying MSC-induced changes in sebocytes and their potential therapeutic applications in skin disorders.