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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-31
Exploration of Endogenous Metabolites Associated with Immune Responses in Bovine Serum
(Osaka Univ.)
oHiroko Kato, Sicheng Tian
In this study, we explored for new endogenous metabolites involved in immune responses. First, bovine serum was fractionated by HPLC and screened, and two fractions that affect immune response were identified. To identify unknown compounds in these fractions, accurate mass measurement and MS/MS spectra were obtained using an Orbitrap mass spectrometer. The elemental composition estimated from the observed accurate masses were searched in the Human Metabolome Database (HMDB), resulting in three candidate compounds (xanthine, oxypurinol, and 6,8-dihydroxypurine) in fraction 1 and one candidate compound (uric acid) in fraction 2. The MS/MS spectra of xanthine, oxypurinol, and uric acid have been recorded in MassBank, and comparison with the MS/MS spectra of the target fractions suggested that fraction 1 is xanthine and fraction 2 is uric acid. The MS/MS spectra of each fraction were also matched with the measured MS/MS spectra of xanthin and uric acid. Furthermore, the activity test using the xanthine and uric acid confirmed the same activity as that of fractions 1 and 2. However, xanthine, oxypurinol, and 6,8-dihydroxypurine have similar structures and are related compounds in the metabolic pathway. Therefore, we analyzed in detail whether xanthine is the active compound in fraction 1.