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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-33
MS Spectrometry based Proteomic Analysis of Bovine Serum for Development of Cell Culture Media
(1KBSI, 2KBSI_2, 3KRIBB, 4UST)
oSoojin Park1, Hae Min Ju2, Jin Young Kim2,3, Heeyoun Hwang2,4
The development of cultured meat aims to replace traditional meat production for environmental sustainability and food security. Fetal Bovine Serum (FBS) is commonly used in culture media due to its essential role in cell proliferation and viability. However, the reliance on imported FBS presents economic challenges, and the increasing cost of FBS hinders the scalability, bovine slaughter byproducts, such as blood, contribute significantly to disposal costs.
To reduce production costs and minimize environmental impact, it is crucial to develop alternative culture media and identify key growth factors. In this study, proteomic analysis was conducted to compare FBS and Adult Bovine Serum (ABS) using LC-MS/MS. Both FBS and ABS were trypsin digested and labeled with TMT 6-plex reagents for quantitative analysis. A total of approximately 300 protein groups and 3,000 distinct peptides were identified using IP2 search based on LC-MS/MS data. Proteins were further analyzed using a t-test to identify common and unique growth factors candidates. The identified growth factors will serve as a foundation for discovering alternative serum-derived components from bovine slaughter byproducts.
This study was supported by the Korea Institute of Marine Science & Technology Promotion (Grant no. RS-2024-00405273)