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Poster Presentations
Day 4, June 25（Wed.）
Room P (Maesato East, Foyer, Ocean Wing)
- 4P-PM-41
Comparison of PEG Precipitation and Ultrafiltration for Serum Exosome Enrichment: Proteomic Analysis Using Mass Spectrometry
(1CNU, 2Chungnam National Univ.)
oHyeongyu Yu1, Jeongkwon Kim2
Exosomes are extracellular vesicles (30–150 nm) that serve as potential biomarkers due to their protein content, which reflects physiological and pathological states. Mass spectrometry (MS)-based proteomic analysis is a valuable approach for characterizing exosomal proteins, but the choice of isolation method can impact protein composition and analytical outcomes. This study compares polyethylene glycol (PEG) precipitation (MW 10,000 and 35,000) and ultrafiltration (Amicon® Ultra Centrifugal Filter, 100 kDa MWCO) for serum exosome enrichment and evaluates their effects on proteomic analysis using Q-Exactive Plus Orbitrap mass spectrometry. Exosomes will be isolated from human serum, and their protein composition will be analyzed using this high-resolution system. Size-Exclusion Chromatography (SEC) and Scanning Electron Microscopy (SEM) will complement MS data by assessing exosome size distribution and morphology. The study aims to determine differences in exosome recovery, protein composition, and purity between these isolation methods. The findings will contribute to optimizing MS-based exosome analysis protocols, ensuring reliable data for biomarker discovery and functional studies.