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ポスター発表

第1日　5月15日（火）　　ポスター会場

1P-51　　PDF

SALSA-glycoblotting法によるスフィンゴ糖脂質糖鎖のシアル酸構造異性体識別

(¹北大院医・ ²島津・ ³創価大理)
^o花松久寿¹・ 西風隆司²・ 三浦信明³・ 岡田和恵¹・ 朴錦花¹・ 坂本直哉¹・ 関谷禎規²・ 岩本慎一²・ 田中耕一²・ 古川潤一¹

Sialic acids exist the nonreducing ends glycans on glcoconjugates with α2,3 and α2,6 linkage. These sialylated glycans are critically involved in many biological events, such as cell adhesion, signal transduction, and virus infection. Glycosphingolipids (GSLs) have a large number of sialyl linked glycan isomers and α2,8 linked polysialic acids. Recently, Nishikaze et al. reported sialic acid linkage-specific alkylamidation (SALSA) method, which allows the discrimination of sialic acid linkage isomers on N-glycans by mass spectrometric analysis. In this presentation, we report that the comprehensive GSL-glycome analysis by SALSA-glycoblotting method combined endoglycoceramidase digestion. Additionally, we introduce the novel method of lactone-opening and amidation with various linear alkyl amines for α2,3 and α2,8 linked sialic acid, which can significantly reduce total reaction time of general SALSA-glycoblotting method.