プログラム

オーラルセッション

第2日　5月16日（水）　18:05～18:20　B会場（星雲1）

2B-O3-M-1805（2P-43）　　PDF

マススペクトルにおけるペプチド同位体比の精確性

(¹京大院薬・ ²京大院工)
^o木部航希¹・ 吉沢明康¹・ 田畑剛¹・ 吉井和佳²・ 石濱泰¹

Isotope distribution of peptide peaks in mass spectra can be used to support the peptide identification, although it has not been so frequently used in proteomics. In this study, we examined the accuracy of the observed isotope distribution of tryptic peptides. We evaluated several factors to influence the similarity between the observed and theoretical isotope distribution. As a result, it was found that the absolute intensity as well as the charge states were the two major factors to affect the accuracy in measuring the isotope distribution of peptides.