- Timetable
- Download all abstracts
- Plenary Lectures
- MSSJ Special Program
- Award Lecture
- Symposium Sessions（Day1, Day2）
- Fundamental Sessions（Day1, Day3）
- Young Researchers' Sessions (Int'l)（Day1, Day3）
- Young Researchers' Sessions（Day1, Day3）
- Poster Presentations（Day1, Day2, Day3）
- Evening Workshop
- Corporate Program
Fundamental Sessions
Day 3, June 12（Wed.） 14:15-14:33 Room B (Convention Hall 200)
- 3B-O2-1415
Development of 13C-Metabolic Flux Analysis of Glycerolipid Metabolism Using Data-Independent Acquisition-Based Lipidomics
(1Osaka Univ., 2Osaka Univ., 3Osaka Univ., 4Shimadzu)
oNobuyuki Okahashi1,2,3, Yuki Yamada1, Daiki Hara1, Atsuhiko Toyama4, Junko Iida2,4, Fumio Matsuda1,2,3
13C-metabolic flux analysis of lipid metabolism was developed using data-independent acquisition (DIA)-based lipidomics. Fragment ions corresponding to glycerol backbones and polar heads was investigated by LC-QTOF/MS. 13C-labeling data of the fragment ions of glycerolipids and phospholipids in budding yeast was analyzed. Flux distribution of a glycerolipid metabolic pathway was successfully estimated by the time-series 13C-labeling data.