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Poster Presentations
Day 3, June 12（Wed.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 3P-30
Glyco-RIDGE-Based Site-Specific Glycoform Analysis of Glycoproteins: Development of a Novel Web Tool Named “GRable" and Application to Large-Scale Analysis of Mouse Tissues
(1AIST, 2Meiji Pharm. Univ., 3Nagoya Univ.)
oChiaki Okatani1, Azusa Tomioka1, Daisuke Tominaga1,2, Hiroaki Sakaue1, Atsushi Kuno1, Hiroyuki Kaji1,3
For effective site-specific glycoform analysis of glycoproteins, we have developed a Glyco-RIDGE method. Here, we describe a novel web tool named GRable for semi-automated Glyco-RIDGE analysis. This tool allows site-specific glycoform analysis of sialic acid-containing intact glycopeptides in a high-throughput manner. We also present a large-scale Glyco-RIDGE analysis of six mouse tissues, in which over 10,000 site-specific N-glycoforms were identified from 800 glycoproteins. Thus, this methodology can be applied to both in-depth analyses of target glycoproteins and large-scale analyses of crude glycoprotein samples, and GRable-assisted glycoproteomics allows for investigation of disease-related glycosylation alterations and thus can facilitate the accelerated development of glycomedicine. This web tool is freely available on the GlyCosmos Portal.