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1E-O5-1545 PDF
1細胞ネイティブ質量分析の開発
Native mass spectrometry (Native MS) provides masses of intact biomolecules in combination with nanoelectrospray ionization (nanoESI), which enables ionization of proteins with retaining non-covalent bonds. In this study, we tried to construct the basis for the “single-cell native MS” method that enables analysis of unpurified proteins directly sampled from a single cell. We modified the “Single-cell mass spectrometry” method, which was originally developed by Masujima et al. for metabolomics study1), and applied it to analyze hemoglobin in a single erythrocyte, as the first step for “single-cell native MS”. By optimizing the experimental conditions, we could successfully observe intact hemoglobin ions by native MS using a single erythrocyte.