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3B-O12-1545 PDF
LC/MS/MSによるHEK293T産生アミロイド前駆体タンパク質770のO-型糖鎖解析
O-glycosylation analysis of amyloid precursor protein 770 (APP770) produced in HEK293T cells was performed by LC/MS/MS of enriched glycopeptides, and 6 O-glycosylated peptide regions (aa269-288, aa352-359, aa364-377, aa378-393, aa649-662, and aa663-670) were successfully identified. Among then, a total of 10 O-glycosylation sites were identified by an additional LC/MS/MS of OpeRATOR-treated glycosylated peptides. Interestingly, the more frequently occurring forms attached to three peptide regions (aa269-288, aa364-377, aa649-662) were Tn (GalNAc) and T (core 1) antigens.