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3C-O13-1525 PDF
レーザーアブレーションICP質量分析計による生体内微量元素とナノ粒子の同時イメージング分析
Metal nanoparticles (MNPs) are widely used in foods and industrial products. With the widespread use of MNPs, the potential risks to human health and the environment are increasingly concerned. Recent research revealed that the toxicity is dependent upon not only the size of MNPs but also ions caused by dissolution of MNPs. Moreover, changes in essential elemental distributions (e.g., Mg, Fe, Cu, or Zn) is another important parameter to evaluate the toxicity of the MNPs. Faced with this, we have developed an analytical technique to define the distribution, size of the MNPs, and elemental concentrations of key nutrients using laser ablation-ICP-time-of-flight mass spectrometry (LA-ICP-TOF-MS). In this study, quantitative imaging analysis of MNPs and biologically relevant elements were conducted on thin section of liver tissues from mice. In-house software and calibration standard material were prepared for a quantitative imaging analysis. With the technique, we can deduce the chemical status of the elements, whether ionic or particulate. The quantitative imaging analysis of essential elements, together with the size analysis of MNPs can provide key information to understand the interaction between MNPs and essential elements.