The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

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Oral Sessions

Day 1, May 19（Wed.）　15:10-15:30　Room A (Zoom)

1A-O1-1510　　PDF

Structural Determination of PK/RiPP hybrid polypeptides using Highly-integrated Radical Induced Dissociation (HiRID)-MS/MS

(1Shimadzu, 2Univ. Tokyo)
oHidenori Takahashi1, Kanae Teramoto1, Shinichi Iwamoto1, Hiroyasu Onaka2, Koichi Tanaka1

In collision-induced dissociation (CID) -MS/MS, which is often used in structural analysis of natural products, rare gases and nitrogen molecules gas are used as collision gases. Our research group has developed a new radical-induced dissociation technique named highly-integrated radical induced dissociation (HiRID) -MS/MS using charge-neutral and highly reactive radicals instead of collision gas. In this study, we succeeded in a structural determination of novel PK/RiPP hybrid polypeptides. The use of HiRID, which integrates a variety of radical species, has made possible the structural analysis of natural products, which has not been possible before.