Oral Sessions (Day1, Day2, Day3)
Oral Sessions
- Day 1, May 19（Wed.） 15:50-16:10 Room A (Zoom)
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1A-O1-1550 PDF
Mass Spectrometric Analysis of Volatile Metabolites in Aspergillus nidulans for Sesquiterpene Biosynthetic Pathway Elucidation Ⅹ. AN3280 Gene Expression-enforced Strains.
Microbial volatile organic compounds (MVOCs) emitted from Aspergillus nidulans were investigated using Head Space-SPME/GC/MS, and the effects of modified expression of some sesquiterpene biosynthesis genes in the genome of A. nidulans were analyzed. In strain AL37, a part of AN3280 gene upstream was eliminated from LO8910 and a rich alcR protein was added from LO8925. The eliminated region seemed to suppress transcription in the parental strain LO8910. We reported previously that there must be intermediates in the production of (+)-aristolochene from farnesyl diphosphate. However, they were not previously detected. In this study we detected a path from farnesyl diphosphate through the intermediates to (+)-aristlochene in A. nidulans.