Oral Sessions (Day1, Day2, Day3)
Oral Sessions
- Day 1, May 19（Wed.） 15:45-16:00 Room E (Zoom)
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1E-O5-1545 PDF
Development of Single Cell Native Mass Spectrometry
Native mass spectrometry (Native MS) provides masses of intact biomolecules in combination with nanoelectrospray ionization (nanoESI), which enables ionization of proteins with retaining non-covalent bonds. In this study, we tried to construct the basis for the “single-cell native MS” method that enables analysis of unpurified proteins directly sampled from a single cell. We modified the “Single-cell mass spectrometry” method, which was originally developed by Masujima et al. for metabolomics study1), and applied it to analyze hemoglobin in a single erythrocyte, as the first step for “single-cell native MS”. By optimizing the experimental conditions, we could successfully observe intact hemoglobin ions by native MS using a single erythrocyte.