Oral Sessions (Day1, Day2, Day3)
Oral Sessions
- Day 3, May 21（Fri.） 15:45-16:00 Room F (Zoom)
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3F-O16-1545 PDF
Simultaneous analysis of All the Aminobutyric Acid Isomers by Chiral Derivatization and LC/MS/MS
There are 8 optical and structural isomers of aminobutyric acid, each of which has a different physiological function. In recent years, some isomers are found to be important for systemic metabolic regulation. However, their detailed metabolism or kinetics in vivo remains unclear since it was difficult to analyze these isomers without interference from not only other aminobutyric acids but also secondary amines, tertiary amines, esters or alcohols with the same chemical formula. Therefore, in this study, we developed a simultaneous analysis method for all aminobutyric acid isomers using an amine-selective chiral derivatization reagent and a C18 column. We first tested 3 chiral derivatizing reagents and found that N-(2,4-Dinitro-5-fluorophenyl)-L-valinamide (FDVA) is better suited for the separation. We then optimized the temperature and time of the FDVA derivatization, and analyzed the level of aminobutyric acids in mouse tissues. All the aminobutyric acids in the tissues were sufficiently resolved and new data regarding tissue-specific metabolism of DL-β-aminoisobutyric acid were able to be obtained.