Abstract

Poster Presentations

Day 3, May 21（Fri.）　　Room P1 (Zoom)

3P-10　　PDF

DIUTHAME offers higher reproducibility than DHB, a widely used matrix in FT-ICR mass spectrometry imaging analysis

(¹HUSM, ²GPI, ³HP K.K, ⁴IMC, HUSM, ⁵SMA)
^oMd. Mahmudul Hasan¹, Fumihiro Eto¹, Md. Al Mamun¹, Shumpei Sato¹, Ariful Islam¹, A.s.m. Waliullah¹, Do Huu Chi¹, Yutaka Takahashi¹, Tomoaki Kahyo¹, Yasuhide Naito², Masahiro Kotani³, Takayuki Ohmura³, Mitsutoshi Setou^1,4,5

DIUTHAME (desorption ionization using through-hole alumina membrane), a recently developed matrix-free ionization-assisting substrate, was examined for reproducibility in terms of mass accuracy and intensity using standard lipid and mouse brain sections. Frozen tissue sections were mounted on indium tin oxide-coated glass slides. DIUTHAME and DHB were applied to individual brain sections. Subsequently, standard phosphatidylcholine, PC(18:2/18:2), was poured onto the DIUTHAME and matrix. Finally, samples were subjected to laser desorption ionization coupled with Fourier-transform ion cyclotron resonance mass spectrometry.
Standard PC(18:2/18:2) analysis showed significantly (p < 0.01) lower mass error for DIUTHAME-MS than MALDI-MS. Endogenous PC(36:4) analysis in mouse brain section also showed significantly (p < 0.05) lower mass errors for DIUTHAME-MS. Furthermore, we investigated the mass error of some abundant lipid ions in brain sections and observed similar results. DIUTHAME-MS displayed lower signal intensity in standard PC analysis. Interestingly, it offered higher signal intensities for all of the endogenous lipid ions.
Our results demonstrated that DIUTHAME-MS offers higher reproducibility for mass accuracies and intensities than MALDI-MS in both standard lipid and mice brain tissue analyses. It can be potentially used instead of conventional MALDI-MS and mass spectrometry imaging analyses to achieve highly reproducible data for mass accuracy and intensity.