The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Society Featured Lecture

Oral Sessions (Day1, Day2, Day3)

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Poster Presentations

Day 3, May 21（Fri.）　　Room P1 (Zoom)

3P-15　　PDF

Expansion the method for metabolite concentration using LC-MS/MS and its application to industrial budding yeast strains.

(1Osaka Univ., 2Kyushu Univ.)
oMasahiko Sugimura1, Nobuyuki Okahashi1, Taisuke Seike1, Yoshihiro Izumi2, Takeshi Bamba2, Fumio Matsuda1

We extended the method for measuring the intracellular concentration of metabolites using LC-MS/MS. In order to expand the target compounds of the reverse labeling method, we attempted to use the LC-MS method with a PFPP column. As a result, we could measure the concentrations of 71 intracellular metabolites out of 91 metabolites that we attempted to analyze. In addition to the glycolytic system, pentose phosphate pathway, and TCA circuit intermediates, the concentrations of amino acids and 32 nucleic acid-related substances were successfully determined.