The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
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Day 3, May 21（Fri.）　　Room P1 (Zoom)

3P-20　　PDF

Application of 13C-Metabolic flux analysis to the oleaginous yeast Yarrowia lipolytica

(1Osaka Univ., 2Kobe Univ.)
oArisa Matsuo1, Nobuyuki Okahashi1, Taisuke Seike1, Chiaki Ogino2, Fumio Matsuda1

The 13C-metablic flux analysis using mass spectrometry was used to investigate metabolic flux distributions in the oleaginous yeast, Yarrowia lipolytica. A time course analysis of cell numbers, medium components and 13C-labeling kinetics of proteinous amino acids and the intracellular metabolites demonstrated that 13C-MFA could be applied for the Y. lipolytica. Flux distributions in the central carbon metabolism suggested that Y. lipolytica during growth phase are specialized both in energy production for cell growth and in production of biomass components.