Abstract

Poster Presentations

Day 3, May 21（Fri.）　　Room P1 (Zoom)

3P-27　　PDF

Glycosylation analysis of glycoengineered biopharmaceuticals produced in silkworms

(¹Yokohama City Univ., ²NARO, ³Osaka Univ.)
^oTakeshi Mukai¹, Kenichiro Tatematsu², Daisuke Takakura¹, Ryou Misaki³, Hiroyuki Kajiura³, Kazuhito Fujiyama³, Hideki Sezutsu², Nana Kawasaki¹

Using LC/MS/MS, we analyzed the glycan structure of rituximab and etanercept produced in transgenic silkworms expressing galactosyltransferase, sialyltransferase and other related enzymes. We found that sialylated and galactosyled hybrid type N-glycans are attached to the transgenic silkworm-derived glycoproteins. Our result suggests that activity of enzymes required for complex type glycan biosynthesis may be lower in silkworms compared to CHO cells.