Abstract

Poster Presentations

Day 3, May 21（Fri.）　　Room P2 (Zoom)

3P-40　　PDF

Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) of Tape-Stripped Human Stratum Corneum in Pursuit of Biomolecule Responsible for Skin Transparency

(¹HUSM, ²KOSE, ³IMIC, ⁴Preppers)
^oA.s.m. Waliullah¹, Takumi Sakamoto^1,4, Tomoya Uchiyama², Eiji Naru², Reimu Fukui¹, Md. Al Mamun^1,4, Tomohito Sato^1,3,4, Yutaka Takahashi^1,4, Tomoaki Kahyo^1,3, Mitsutoshi Setou^1,3,4

Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) is a label-free and rapid molecular imaging technique in situ. The straightforward sample preparation protocol made DESI-MSI a powerful tool in analyzing some tissue region, for example, skin surface, from where sample collection is challenging for other MSI techniques. Tape stripping of skin combined with DESI-MSI is a promising high throughput biomolecular analysis technique. Here, we aimed to apply DESI-MSI to tape-stripped human stratum corneum (SC) samples to explore biomolecules responsible for skin transparency. We have collected SC samples by tape stripping from the cheek of 11 healthy female subjects with the skin transparency parameters. DESI-MSI analysis was conducted using Xevo G2-XS Q-ToF mass spectrometer(Waters, USA)in positive ion mode. We specifically detected molecule “X" in the subject with the highest skin transparency. Currently, we are working on molecular identification using LC-MS/MS and analysis tools such as Metfrag, a tandem mass spectrometry annotation tool. Identification of the molecule “X" will potentially lead us to establish the scientific basis for skincare product development using mass spectrometry.