Oral Sessions (Day1, Day2, Day3)
Poster Presentations
- Day 3, May 21（Fri.） Room P2 (Zoom)
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3P-49 PDF
Improvement of the Method for identifying Neoantigens in HCT116 cells
Recently, cancer neoantigens have been paid great attention as a cancer vaccine target. Neoantigens are self-peptides that are displayed on major histocompatibility complexes (MHC), carrying somatic mutations that are observed only in cancer tissues. When we searched for MHC-associated neoantigens by a mass spectrometry, we faced a problem to identify these peptides because they are buried in the noise in the biological sample. We improved the peptide separation by using the nanocapillary column (Aurora column) so that more MS/MS spectra could be obtained. Because the column has a high resolution of 1.6µm particle and designed to have a low dead volume, samples can be sprayed efficiently into the mass spectrometer. As a result, we were able to identify many peptides that could not be identified before.
Peptides were extracted from cultured HCT116 colon cancer cells, immunoprecipitated with anti-MHC antibody, and measured by a mass spectrometer. Peptide sequences were searched against database obtained from the exome and transcriptome from HCT116. Currently we are in the process of searching for neoantigens from clinical tumor samples.