The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

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Day 3, May 21（Fri.）　　Room P2 (Zoom)

3P-50　　PDF

Improvement of proteomics assay for central carbon metabolism in human breast cancer cells

(1Osaka Univ., 2Niigata Univ.)
oRyo Takahashi1, Kazuki Nishimoto1, Nobuyuki Okahashi1, Masaki Matsumoto2, Fumio Matsuda1

The selection of precursor and product ions with high sensitivity and specificity is important for protein quantifications by Multiple Reaction Monitoring (MRM) assay. We optimized peptide selection and the number of product ions detected for each peptide. As a result, two more enzymes for central carbon metabolism in human breast cancer cell were successfully quantified, suggesting that selecting peptides with appropriate weights and analyzing more than 5 product ion per peptide are suitable for MRM assay.