The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Society Featured Lecture

Oral Sessions (Day1, Day2, Day3)

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Poster Presentations

Day 3, May 21（Fri.）　　Room P2 (Zoom)

3P-65　　PDF

13C-Metabolic Flux Analysis of THP1 Before and After Differentiation by Using Mass Spectrometry

(Osaka Univ.)
oMana Hontani, Takeo Taniguchi, Nobuyuki Okahashi, Fumio Matsuda

13C-metablic flux analysis (MFA) using mass spectrometry was used to investigate metabolic flux distributions in differentiated and non-differentiated THP1 monocyte leukemia cells. A time course analysis of cell numbers, medium components and 13C-labeling kinetics of the intracellular metabolites confirmed that 13C-MFA could be applied for the immune cells. Flux distributions in the central carbon metabolism showed that the NADPH regeneration rate was increased by 7-fold in differentiated THP1 cells compared with non-differentiated ones, suggesting that metabolism of differentiated THP1 was rewired for immune responses.