The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Society Featured Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day3)

Workshops

Lunchtime Seminars (Day1, Day2, Day3)

Poster Presentations

Day 3, May 21（Fri.）　　Room P2 (Zoom)

3P-66　　PDF

Development of isotopically nonstationary 15N-metabolic flux analysis for nucleotide metabolism

(Osaka Univ.)
oKeita Murakami, Yuya Kondo, Nobuyuki Okahashi, Fumio Matsuda

Isotopically nonstationary 15N-meatabolic flux analysis (INST 15N-MFA) using mass spectrometry was developed for investigating nucleotide metabolic flux distribution in colon cancer cell lines. Time-course data of cell numbers, medium components and 15N-labeling kinetics of the intracellular metabolites successfully estimated the nitrogen flux through amino acid and nucleotide metabolism.