The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 2, June 23（Thu.）　15:20-15:40　Room A (Main Hall)

2A-O2-1520　　PDF

Nano-LC/MS/MS-based analytical system for single-cell analysis

(1Kyushu Univ., 2AIST, 3Niigata Univ.)
oYoshihiro Izumi1, Kosuke Hata1, Kohta Nakatani1, Shohei Yamamura2, Masaki Matsumoto3, Takeshi Bamba1

Analytical methods to accurately identify and quantify comprehensive metabolites or proteins from single mammalian cells are still in development. The aim of this study was to develop a single-cell metabolomic and proteomic analytical system based on highly sensitive nano-liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). We firstly developed a method called in-line sample preparation for efficient cellular metabolomics or proteomics (ISPEC). By combining live single-cell sampling, ISPEC, and nano-LC/MS/MS, we successfully detected approximately 50 metabolites or 300 proteins from single cells. Our single-cell proteomic and metabolomic analytical system represents a potentially useful tool for in-depth studies focused on cell metabolism and heterogeneity.