The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 2, June 23（Thu.）　15:40-16:00　Room A (Main Hall)

2A-O2-1540　　PDF

Mass Spectrometry Imaging of Dilated Cardiomyopathy Tissues by Tapping-mode Scanning Probe Electrospray Ionization

(1Osaka Univ., 2JST・PRESTO, 3Osaka Univ., 4Osaka Univ.)
oYoichi Otsuka1,2, Shuichi Shimma3, Hidetaka Kioka4, Tomohito Ohtani4, Yasushi Sakata4

Mass spectrometry imaging (MSI) is a technique to measure the multidimensional chemical information to visualize the distribution of components in samples. We have been developing tapping-mode scanning probe electrospray ionization (t-SPESI) and applying it to MSI of biological tissues. In this presentation, we will report the results of high mass resolution MSI of human diseased heart tissues using a t-SPESI system with a built-in feedback control device. The MSI results showed that there were multiple lipids whose ion intensities were distributed throughout the tissue, as well as lipids localized within the tissue. The localized lipids were tentatively assigned as triacylglycerols (TG). The localization information of TG species in cardiac tissues may lead to the stratification of pathophysiology and the prognosis prediction of dilated cardiomyopathy. We showed that MSI by t-SPESI to measure the distribution of multiple TGs, which is difficult to achieve using staining methods.