The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 2, June 23（Thu.）　16:00-16:20　Room B (409 and 410)

2B-O2-1600（2P-35）　　PDF

High-throughput Analysis of Anthocyanins in Horticultural Crops by PESI/MS/MS

(1Nagoya Univ., 2Kyoto Univ., 3Kindai Univ.)
oMisaki Ishibashi1,2, Ikue Yoshikawa1, Kei Zaitsu3, Akira Oikawa2, Shungo Otagaki1, Shogo Matsumoto1, Katsuhiro Shiratake1

Secondary metabolites are important for traits of horticultural crops; color, aroma, taste and functionality. High-throughput analysis of these metabolites have been required at breeding field, distribution and consumer market. We applied probe electrospray ionization (PESI) to evaluate the quality of plants, especially horticultural crops. PESI does not require pre-treatment and separation of samples, and so it is suitable for high-throughput analysis. In this study, we targeted anthocyanin, one of the most major pigments in fruit and vegetable. Nearly 70 kinds of anthocyanins were detected in approximately 3 min at selected reaction monitoring mode. Tandem mass spectrometer (MS/MS) could distinguish the fragments of anthocyanin and flavonol, adequately. Probe sampling, the simpler method sticking a probe directly to sample, could detect anthocyanins specifically in micro area scale. In strawberry, fruit including seed (achene) and pulp (receptacle) accumulated different molecular species of anthocyanins. PESI/MS/MS could be utilized to characterize the profile of anthocyanins, and to compare the contents among cultivars quantitatively.