Abstract

Oral Sessions

Day 3, June 24（Fri.）　15:00-15:20　Room A (Main Hall)

3A-O2-1500　　PDF

Structural library and visualization of endogenously oxidized glycerophospholipids using mass spectrometry-based techniques

(Kyushu Univ.)
^oYuta Matsuoka

Although oxidized glycerophospholipids (oxGPLs) play critical roles in numerous pathological events, the type and production sites of endogenous oxGPLs remain unknown because of the lack of structural information and dedicated analytical methods.
Herein, a library of 465 oxidized phosphatidylcholines (oxPCs) is constructed using high-resolution mass spectrometry-based non-targeted analytical methods (Figure 1) and employed to detect 70 oxPCs in mice with acetaminophen-induced acute liver failure1). We show that doubly oxygenated polyunsaturated fatty acid (PUFA)-PCs (PC PUFA;O2), containing epoxy and hydroxide groups, are generated in the early phase of liver injury. Hybridization with in-vivo 18O labeling and matrix-assisted laser desorption/ionization-tandem MS imaging reveals that PC PUFA;O2 are accumulated in cytochrome P450 2E1-expressing and glutathione-depleted hepatocytes, which are the major sites of liver injury. The developed library and visualization methodology should facilitate the characterization of specific lipid peroxidation events and enhance our understanding of their physiological and pathological significance in lipid peroxidation-related diseases.