The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 3, June 24（Fri.）　14:40-15:00　Room B (409 and 410)

3B-O2-1440　　PDF

Applications and Challenges in Mass Spectrometry-Based Proteomics for Host Cell Protein Assay

(Natl. Inst. Health Sci.)
oMasashi Hyuga, Minoru Tada, Akiko Ishii-Watabe

Host Cell Proteins (HCP) are process-related impurities of biopharmaceuticals. The enzyme-linked immunosorbent assay using anti-HCP polyclonal antibody (HCP-ELISA) is gold standard method for quantitative HCP analysis. In recent years, proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is applied as an important orthogonal method for HCP analysis. LC-MS/MS enables identification and quantitation of individual HCPs. In this presentation, we will discuss the applications and challenges toward implementation of LC-MS/MS for HCP analysis.