The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 3, June 24（Fri.）　15:20-15:40　Room B (409 and 410)

3B-O2-1520　　PDF

Development and physicochemical properties of monoclonal antibody reference material, AIST-MAB

(AIST)
oTomoya Kinumi, Kazumi Saikusa

We have developed a monoclonal antibody reference material, NMIJ 6208a, AIST-MAB to address extensive needs for antibody characterization. The material is a humanized IgG1κ in phosphate buffer solution, whose protein concentration was precisely determined by amino acid analyses using isotope-dilution mass spectrometry. We also summarized the results of measurements of various properties including peptide mapping. This material can be used as a reference for antibody concentration, while provides a tool that enables a wide range of analytical techniques to be extensively compared and evaluated, going beyond quality control with in-house reference material. This material and the measured properties as the case study are currently available from NMIJ/AIST.