The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 3, June 24（Fri.）　10:20-10:40　Room C (411 and 412)

3C-O1-1020　　PDF

Profiling Triglycerides by Liquid Chromatography-Multiple Reaction Monitoring-Mass Spectrometry

(1Univ. Tokyo, 2Shimadzu)
oYoshihiro Kita1, Suzumi Tokuoka1, Doriane Toinon2, Masaki Yamada2, Yoshiya Oda1

Triglycerides have three esterified fatty acyls on a glycerol backbone and there are many different molecular species depending on fatty acyl diversity and their bonding positions. In the present report, we tried to profile blood triglycerides using wide-target LC-MRM-MS. Triglycerides were detected by MRM transitions based on the known fragmentation at fatty acyl groups. A preliminary analysis was performed using a large set of MRM transitions generated by a combination of fatty acyls, and MRM channels with signals were screened and put into a single measurement method. Real samples were analyzed using the method, and the peak areas for each MRM channel were summarized and graphed at the species level or at the level of molecular species with one fatty acid identified. Since the construction of such an analytical method is a routine work that is repeated each time the target sample and/or measurement conditions are changed, it is desirable to perform the process systematically, reliably, and quickly. We would like to summarize and discuss the experience in this study on avoiding misidentification during MRM channel screening by isotope correction using TRACES, a tool we developed, as well as managing MRM panels and improving routine data analysis using R.