Oral Sessions (Day1, Day2, Day3)
Poster Presentations (Day1, Day2, Day3)
Oral Sessions
- Day 3, June 24（Fri.） 10:00-10:20 Room D (413 and 414)
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3D-O1-1000 PDF
Laser Desorption/Ionization Mass Spectrometry Using Nanoporous Organosilica Substrates with Hydrophobic/Hydrophilic Mixed Surface
The rapid detection of bio-related compounds by mass spectrometry (MS) has been expected to contribute to clinical diagnosis and health management. In this study, UV-absorbing nanoporous organosilica films have been developed as analytical substrates for laser desorption/ionization mass spectrometry (LDI-MS) of peptides and small proteins. Vertically oriented nanopore arrays are formed by nanoimprinting of naphthalimide-containing organosilica films. The surface of the nanoporous substrates is functionalized with perfluoroalkyl/alkylamide groups mixed at a molecular level. This surface contributes to both lowering the surface free energy and introducing weak anchoring sites for peptides and proteins. Peptide molecules applied onto the substrates are homogeneously distributed due to the effect of the alkylamide groups, and readily desorbed by the laser irradiation due to the low surface energy of the substrates. An amyloid β protein fragment, which is known as a biomarker for Alzheimer's disease, can be detected at a low concentration (50 pM) comparable to that in blood. The detection of the amyloid β at 200 pM is also confirmed in the presence of blood components. The molecular-level surface design of the nanostructured organosilica substrates may enable easy detection of a wide range of biomolecules by LDI-MS.