The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Award Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Panel Discussion

Luncheon Seminars (Day1, Day2, Day3)

Corporate Program

Oral Sessions

Day 3, June 24（Fri.）　10:20-10:40　Room D (413 and 414)

3D-O1-1020　　PDF

Development of semi-quant metabolomics workflow for human plasma samples by GC/MS with a calibration-curve-locking database (CCLD)

(1Agilent, 2Sumitomo Chemical, 3Nagoya Univ., 4Tokyo Univ. Agr. Tech., 5FRA, 6Kyushu Univ.)
oAya Anegawa1, Fukumatsu Iwahashi2, Jun Ueyama3, Yoshimasa Tsujii4, Takeshi Hano5, Kosuke Hata6, Yuki Soma6, Toshiyuki Yamashita6, Yoshihiro Izumi6, Takeshi Bamba6

We developed the semi-quant metabolomics workflow, which includes about 90 calibration curves for human plasma samples by GC/MS. In this study, we examined the possibility of semi-quantification screening using a calibration-curve-locking database (CCLD) for the concentration of hydrophilic metabolites in human plasma, NIST SRM1950, without requiring calibration curve as compared to 2019 and 2021 data sets. As a result of these examinations, the workflow and semi-quantification we developed using CCLD methods suggest to be effective in metabolomics for human plasma samples.