演題概要

ポスター発表

第1日　6月22日（水）　　P会場（501，502，503）

1P-07　　PDF

アルカリ金属イオン付加体のMS² を利用したペプチドの配列解析

(¹広大院統科・ ²島津)
齋藤遥平¹・ ^o山口愛歩¹・ 福山裕子²・ 田中耕一²・ 泉俊輔¹

In matrix-assisted laser desorption/ionization (MALDI), peptides are often detected as [M+H]⁺, but they can also be observed as alkali metal adducts, such as [M+Na]⁺. In this study, MS² of alkali metal ion adducts ([M+Li]⁺, [M+Na]⁺, [M+K]⁺, [M+Rb]⁺ and [M+Cs]⁺) and [M+H]⁺ for peptides were compared. As a result, MS² fragments for all the alkali metal ion adducts were mainly detected as y-ions or b-ions. In particular, ratio of total peak intensity of y-ions to total peak intensity of b-ions was highest in the [M+Cs]⁺ MS² spectrum.