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2A-O2-1440 PDF
Proteomics Study Reveals Mechanism of Radiation-Induced Fibrosis Ameliorated by Algae Extract
Fucoidans, sulfated and fucosylated polysaccharides extracted from brown seaweed, were found to inhibit radiotherapy-induced cell damage and fibrosis through the TGF-β1 pathway. However, the comprehensive molecular response during irradiation-induced fibrosis and fucoidan-assisted recovery still remain unclear. Rat hind limbs were irradiated and smeared with low molecular weight fucoidan (LMF). Protein profiles were examined by a mass spectrometry-based proteomics analysis. Out of a total of 4,625 proteins, 233 were found to be significantly up-regulated after irradiation and down-regulated after LMF treatment. Pathway and protein-protein interaction network analyses further indicated that seven proteins including Actb, Ezr, Msn, Cdc42, Actr3, Arpc2, and Cfl1 were clustered into the tight junction and regulation of actin cytoskeleton pathways. These seven proteins may serve as biomarkers for the detection of skin fibrosis induced by irradiation and for the recovery following LMF treatment.