演題概要

ポスター発表

第3日　6月24日（金）　　P会場（501，502，503）

3P-06　　PDF

誘導体化による一細胞内アミノ酸の高感度質量分析法の開発

(¹静岡県大薬・ ²味の素)
^o水野初¹・ 西島公佳¹・ 山口侑季乃¹・ 唐川幸聖²・ 原田真志²・ 杉山栄二¹・ 中山聡²・ 轟木堅一郎¹

In this study, we developed a highly sensitive analytical method by introducing a derivatization method to live single-cell mass spectrometry to detect intracellular amino acids. Trace amounts of amino acid standards were derivatized with (R)-BiAC reagent in a glass capillary for nanoESI, and mass spectrometric analysis showed improved peak intensities for all amino acids. Furthermore, this method was applied to single-cell analysis, and derivative peaks of all 20 amino acids in the cell could be detected at the single-cell level.