基盤セッション
- 第1日 5月15日（月） 10:54～11:12 A会場（特別会議場）
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1A-O-1054 PDF
溶媒抽出表面分析法とナノ流量LC/イオンモビリティーMSを用いたマウス脳における糖脂質異性体のイメージング
Glucosylceramide (GlcCer) is degraded by β-glucocerebrosidase (GBA). Heterozygous mutations in lysosomal GBA (GBA1) gene are the most potent risk factor for Parkinson’s disease (PD). GBA1 heterozygous mutations reduce not only GBA1 activity but also non-lysosomal GBA (GBA2) activity. Previously, we demonstrated an existence of sterylglycosides (SGs) in vertebrate brain for the first time. We demonstrated that GBA1 and GBA2 possess not only GlcCer hydrolase activity but also SG synthesizing and degrading activities. To elucidate the involvement of SGs in PD, it is important to understand the localization of SGs in brain. However, localization analysis is not possible because there are no probes or antibodies that specifically recognize SGs. In this study, we tried to develop an analytical platform for analyzing the localization of SGs using MS. Since SGs include isomers, it is difficult to distinguish each other by traditional MS. Here, we report the development of an isomeric glycolipid imaging system using robotic liquid extraction surface analysis (LESA) coupled with nano-flow LC/ion mobility-MS. Using our system, we could detect SG isomers separately in a mouse brain section.