ポスター発表
- 第1日 5月15日（月） P会場（ホワイエ，会議室1004-1007）
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1P-28 PDF
質量分析計（MALDI TOF MSとESI MS）によるO結合型糖鎖誘導体のイオン化能の比較
Glycoprotein glycans are mainly classified into two types, N-glycans and O-glycans, according to their linkage on the proteins. In particular, since O-glycans play important biological and medical roles, the structural analysis of O-glycan is necessary to elucidate their functions. At present, structural analysis of glycans based on mass spectrometers with high sensitivity and structural analysis capability is commonly used. However, since glycans are generally characterized as neutral molecules, they cannot be detected with high sensitivity by mass spectrometry because of their low ionization efficiency of native glycans. Therefore, labeling or derivatization to improve ionization efficiency is essential for structural analysis of glycans based on mass spectrometers. Recently, a labeling agent with high ionization efficiency for N-glycans have been developed and commercially available, but it is very difficult to use this agent for O-glycans due to the differences in functional groups (ref). We are developing O-glycan labeling agents with high ionization capacity to analyze O-glycans by mass spectrometry. As a part of this research, the ionization capacities of 2-aminobezamide (2AB), procainamide (ProA), 1-phenyl-3-methyl-5-pyrazolone (PMP), which are commonly used as labeling agents for O-glycans using mass spectrometers (MALDI-TOF MS and ESI-MS) were evaluated, and the results are reported.