ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
-
2P-01 PDF
MALDI-MSにおけるペプチド分析のための新規マトリックスalkylated hydroxy chalcone
In matrix assisted laser desorption/ionization mass spectrometry, matrices more suitable for analytes are selected. Matrices such as alkylated trihydroxy acetophenone (ATHAP) with alkyl chains are suitable for hydrophobic peptide analysis 1). In this study, alkylated hydroxy chalcone (AHC), a compound with alkyl chains like ATHAP, was used as a matrix, and hydrophobic peptide humanin was detected as [M+H]+ from outer edges of a matrix/analyte crystal spot. However, the poor crystallinity of AHC may reduce reproducibility. In order to overcome this weakness, AHC was mixed with 2, 5-dihydroxy benzoic acid (2, 5-DHB). In addition, AHC with a high absorbance at 355 nm was expected to improve the sensitivity. As a result, when a 9:1 (v/v) mixture of 2, 5-DHB and AHC was used as a matrix, hydrophobic peptides ions were detected with higher sensitivity.