ポスター発表
- 第2日 5月16日（火） P会場（ホワイエ，会議室1004-1007）
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2P-02 PDF
体外嫌気性-好気性共培養モデルにおける Clostridioides difficile 感染に対する宿主応答のマルチオミックス解析
Clostridioides difficile, a type of enteric pathobiont, has the potential to cause severe health consequences such as toxic megacolon, intestinal perforation, and even death. Despite the seriousness of this infection, there has been limited research on how the bacteria interact with host cells and the host’s response at the molecular level. To address this gap, we have developed an in vitro co-culture model that recapitulates C. difficile infection and allows for direct interaction between the bacteria and human gut epithelial cells under anaerobic-aerobic conditions. Our model was validated by observing toxin-mediated phenotypes such as the disruption of the intestinal epithelial barrier and disaggregation of actin filaments. We used an integrative multiomics approach to comprehensively investigate the biological changes and response mechanisms of host cells in the early stages of C. difficile infection. Our finding suggests an increase in stress-induced chaperones and activation of the ubiquitin proteasomal pathway, while proteins involved in the electron transfer chain and ATP synthase were reduced. Furthermore, the metabolomic analysis revealed suppression of energy metabolism (glycolysis and the TCA cycle). Therefore, this study provides valuable insight into the molecular-level pathway of host cell response to C. difficile infection under anaerobic-aerobic conditions.