ポスター発表
- 第2日 5月16日(火) P会場(ホワイエ,会議室1004-1007)
-
2P-47 PDF
タンデム質量分析計でのDIA測定によるリボヌクレアーゼ消化mRNA の自動評価アルゴリズム
The demand for the characterization of modified messenger (m)RNA is increasing with the development of mRNA therapeutics. An effective experimental technique for mRNA characterization is digestion with sequence-specific ribonuclease, such as RNase T1, followed by analysis of the digest by liquid chromatography (LC)-tandem mass spectrometry (MS/MS). Although digests are preferably analyzed one by one by data-dependent MS/MS acquisition (DDA), in such experiments some nucleotide ions are often missed by chance. In contrast, data-independent MS/MS acquisition (DIA) experiments allow all detected nucleotide ions to be captured in a one-shot analysis; however, there is no software tool for DIA data of RNA. Here, we propose a new algorithm for the characterization of long, modified RNAs using DIA MS/MS data.