日本質量分析学会 第71回質量分析総合討論会

演題概要

シンポジウムセッション

第3日 5月17日(水) 14:15~14:37 A会場(特別会議場)

プロテオーム試料を基質とした酵素反応における基質タンパク質構造の影響

(1京大院薬2医薬基盤健栄研)
o小形公亮1前田朝登1村松邑香1杉山直幸1石濱泰1,2

Enzymatic reactions have been used as tools to analyze proteomic samples. Proteases are employed to digest large proteins into smaller peptides. Protein phosphatases are utilized to remove phosphate groups from proteins, while protein kinases are utilized to add phosphate groups to target proteins. Recently, limited proteolysis has been utilized to map substrate structural changes as it preferentially cleaves surface-exposed domains of proteins. Motivated by this, we conceptualized the use of various enzymes for conformational mapping. We examined the effects of substrate structure on the efficiency of limited reactions with protease, kinase, and phosphatase. As a result, all three enzymes preferred the residues which are more exposed, supporting the possibility of structural profiling of substrate proteins with these enzymatic reactions.